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Evans Blue Dye (T-1824) is a di-azo compound and has been the principal method of determining blood volume
in humans and animals for over eighty years. The first paper was published in 1915 and we have over 60 other publications listed, all specifically referencing Evans blue dye for blood volume studies.
Evans blue dye is an important perameter in cardiovascular physiology. Several normal and pathological conditions affect blood
volume along with the many physiological mechanisms which are involved in the regulation of plasma volume. Plasma volume determination therefore, and in particular repeated determinations, are of interest in many
physiological studies and clinical situations.
The dye combines firmly with plasma albumin when injected into the blood
stream and which leaves the circulation very slowly. The dye is removed from the vascular system principally by diffusion into the extra vascular tissues while still bound to albumin. It is also taken up in small
amounts in the bile and by wandering phagocytic cells.
Numerous papers have been published on methodology and we supply the latest computer technique, a programme for blood volume determination using EB and multiple sample technique. Knowing the amount of dye
injected, times of blood sample collections, and spectrophotometer readings for the samples, the program (1) automatically corrects each sample for its own blank value, (2) finds the best EB disappearance curve, (3)
back-calculates to injection time, (4) corrects for preceding dye concentration and if the hematocrit is known, (5) returns the plasma volume.
SPECIFICATION; A complete specification of the product.
PAPERS: Over 60 specialist papers on the use of Evans blue.
METHODOLOGY; Two methods, including our computer based model.
CONTACT US: E-mail for prices and availability.
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